Thiago Vivacqua
Western University, CanadaTitle: Protocol for harvest, transport and storage of human osteochondral tissue
Abstract
Osteochondral fragments measuring 2 x
3 cm of 5 donors aged between 15 and 45 years old were analyzed. The samples
were stored in a cell preservation medium contain human albumin, Iscove’s, and
vancomycin preserved at 4°C. The concentration of proteoglycans in the
extracellular medium was quantified with Safranin-O, while tissue structural
analysis was assessed by histological study with hematoxylin-eosin-stained
slides. The images obtained were analyzed according to the histological scores
of Mankin and the score proposed by the Osteo Arthritis Research Society
International. The samples were analyzed with 0, 15, 30, and 45 days of
preservation. The osteochondral fragments studied showed a progressive
decrease in proteoglycan concentration with increased preservation time. After
30 days of preservation, structural changes were identified with discontinuity
of the cartilage surface layer. According to the results obtained by the
Mankin score, there was a statistically significant difference between 15 and
30 days of tissue preservation. The protocol described defined knee
transport immersed in Lactated Ringer at a controlled temperature of 10°C until
it arrives at the tissue bank. The preservation solution is composed of
Iscove’s serum-free cell culture medium supplemented with 10% human albumin and
100 ?g/ml vancomycin. The tissue is preserved at a temperature of 4°C until the
moment of transplantation characterizing the fresh preservation.
Biography
Thiago Vivacqua has completed his MD at the
age of 35 years from Rio de Janeiro, Brazil. He is a Knee Research Fellow at
Fowler Kennedy Sport Medicine Clinic, Western University, London, Ontario, CA. The
submitted abstract was developed at the National Institute of Orthopedics
Surgery, Rio de Janeiro, Brazil.
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