Thiago VivacquaWestern University, Canada
Title: Protocol for harvest, transport and storage of human osteochondral tissue
Osteochondral fragments measuring 2 x 3 cm of 5 donors aged between 15 and 45 years old were analyzed. The samples were stored in a cell preservation medium contain human albumin, Iscove’s, and vancomycin preserved at 4°C. The concentration of proteoglycans in the extracellular medium was quantified with Safranin-O, while tissue structural analysis was assessed by histological study with hematoxylin-eosin-stained slides. The images obtained were analyzed according to the histological scores of Mankin and the score proposed by the Osteo Arthritis Research Society International. The samples were analyzed with 0, 15, 30, and 45 days of preservation. The osteochondral fragments studied showed a progressive decrease in proteoglycan concentration with increased preservation time. After 30 days of preservation, structural changes were identified with discontinuity of the cartilage surface layer. According to the results obtained by the Mankin score, there was a statistically significant difference between 15 and 30 days of tissue preservation. The protocol described defined knee transport immersed in Lactated Ringer at a controlled temperature of 10°C until it arrives at the tissue bank. The preservation solution is composed of Iscove’s serum-free cell culture medium supplemented with 10% human albumin and 100 ?g/ml vancomycin. The tissue is preserved at a temperature of 4°C until the moment of transplantation characterizing the fresh preservation.
Thiago Vivacqua has completed his MD at the age of 35 years from Rio de Janeiro, Brazil. He is a Knee Research Fellow at Fowler Kennedy Sport Medicine Clinic, Western University, London, Ontario, CA. The submitted abstract was developed at the National Institute of Orthopedics Surgery, Rio de Janeiro, Brazil.