Abdelouahab DiafatUniversity El-Bachir El-Ibrahimi, Algeria
Title: In vivo and in vitro anti-inflammatory and anti-arthritic effect of Peganum harmala
Medicinal plants are potential sources of natural products that play an important role in preventing different human diseases; the aim of the present study is to evaluate the anti-inflammatory and the anti-arthritic effect of Peganum harmala L. The anti-inflammatory effects were evaluated in vitro by bovine serum albumin (BSA) denaturation inhibition test and in vivo using xylène-induced ear edema model. Arthritis was induced in rats by intradermal immunization with type II collagen emulsified in Freund’s Adjuvant, then arthritic rats were treated with P. harmala hydromethanolic extract (PhHm) for 21 days and for 40 days for preventive and curative study, respectively, using two doses (50 and 100 mg/kg/Day). Results showed that PhHm extract significantly inhibited the BSA denaturation. It had also a good anti-inflammatory effect in xylene-induced ear edema model. P. harmala extract significantly inhibited arthritic development. Treatment with the plant extract also nearly normalized counts of platelets and total leukocytes. In conclusion, the current study demonstrated that the hydromethanolic extract of P. harmala plays an effective role in the anti-inflammatory effect and in both prevention and curative treatment of collagen-induced arthritis.
Keywords: Rheumatoid arthritis, type II collagen, Peganum harmala L., anti-inflammatory, anti-arthritic.